DNA

Part:BBa_K315011:Design

Designed by: Anvi Raina   Group: iGEM10_Davidson-MissouriW   (2010-06-24)


Variant reverse lox N


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

In constructing variant lox sites, we looked for the least promiscuous sites - those that had the lowest rates of recombination with other variants.


Source

We learned about the use of variant lox sites such as lox N to produce random fluorescent protein expression from the "Brainbow" paper (http://www.nature.com/nature/journal/v450/n7166/full/nature06293.html).

References