DNA
Part:BBa_K315011:Design
Designed by: Anvi Raina Group: iGEM10_Davidson-MissouriW (2010-06-24)
Variant reverse lox N
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
In constructing variant lox sites, we looked for the least promiscuous sites - those that had the lowest rates of recombination with other variants.
Source
We learned about the use of variant lox sites such as lox N to produce random fluorescent protein expression from the "Brainbow" paper (http://www.nature.com/nature/journal/v450/n7166/full/nature06293.html).